Category Archives: 3D Spheroids

Wounding, Interleukin-1 and Other Hair News

There were way too many developments in the past month, at least half of which probably deserved their own post.

Wounding, Interleukin-1 (IL-1) and γδ T-cells

Wounding induced hair growth has been in the news a lot over the past several years.

At the end of 2013, I first discussed Dr. Krzysztof Kobielak and Dr. Eve Kandyba from USC in relation to their work on how the Wnt7 gene activates hair growth. Fast forward to this month, and both scientists are back in the news and (via a collaboration with researchers from UC San Diego and from India) published an important paper titled: “Stimulation of hair follicle stem cell proliferation through an IL-1 dependent activation of γδT-cells“.

Interestingly, one of the three most important words used throughout the paper and of primary importance to us (“wounding” or “wound“) is missing from the above title, although not from the complementing photo (see red area in the image further below).

These scientists found that wounding triggers interleukin-1 (IL-1), which in turn activates certain immune cells (called γδT-cells or gamma delta cells). These immune cells then awaken resting stem cells in the hair follicle so that they can then “multiply and travel to the wound site to repair the injury”. For our purposes, the hair follicle stem cell activation is more important than any kind of wound repair (i.e., hair > scar improvement).

Commentator “Omg” who has posted some great material in recent weeks (including the above — thanks) wrote that this finding is the biggest discovery of the past decade. I am not so sure. Note that the authors of this latest work are not affiliated with Follica (the widely discussed company that is developing a “wounding + favorable compound injection” related procedure to regrow hair); nor are they affiliated with Dr. Rachita Dhurat, who has published several groundbreaking papers related to wounding and hair growth. Makes this discovery from an entirely different group all the more exciting, and further validates the potential of wounding spurred hair regrowth.

Wounding, Interleukin-1 and Hair Growth

Nanogel Encapsulated Dermal Papilla 3D Spheroids and Hair Follicle Regeneration

In 2015 I interviewed Dr. Malcolm Xing from Canada. In 2016, I discussed the same doctor’s new findings related to a novel improved “hanging drop” 3D cell culturing technique for the purpose of hair growth. Dr. Xing has constantly been trying to improve upon the renowned Dr. Colin Jahoda’s work.

This month, Dr. Xing and a team from China have published a new paper titled “Bottom-up Nanoencapsulation from Single Cells to Tunable and Scalable Cellular Spheroids for Hair Follicle Regeneration“.

I am not motivated enough to try to make the sci-hub site work to gain free access to this whole paper. I am, however, in no doubt that any new development related to 3D spheroids and hair culturing is of utmost importance and warrants coverage on this blog.

More Good News from Aclaris

In November, I discovered an interview in which Aclaris Therapeutics’ chief scientific officer mentioned that in the  first half of 2018, Aclaris planned to commence phase 2 trials to treat androgenetic alopecia (male pattern hair loss) with topical JAK inhibitors. This was a huge development, since we are used to virtually all companies in the hair loss world postponing or delaying clinical trials. In contrast, Aclaris is doing the opposite and moving to phase 2 trials without even having done phase 1 trials! They probably got to skip phase 1 trials because they have tested the same JAK inhibitors (including oral and topical versions) for other conditions.

Several weeks after I posted the above pleasing development, Aclaris announced at an investor conference that they would start the above trials at the end of the first quarter of next year (i.e, end of March 2018). Yet again they surprise us with their speed, and do not want to wait will June 2018. The announcement can be accessed by going to their website and finding their latest investor conference presentations (often available in both audio and slide formats).

Thanks to “Royaume” who listened to the whole audio for us (albeit misinterpreted its favorable implication). Commentator “Malcolm” even felt like I should have devoted a whole post to this news, but I did not want to do so less than a month after my prior post on Aclaris. Moreover, past experiences make me think that Aclaris will delay its trial commencement target dates (like all other companies seem to do), although I hope I am wrong.

Replicel CEO Update

Replicel’s CEO was interviewed by “Investing News” towards the end of last month. Key quote:

“There’s a possibility… that [our] hair products could have an early launch in Japan. Our partner Shiseido is funding a clinical trial in Japan that’s expected to release clinical data next year. It’s entirely up to Shiseido what they do in regards to this product. There’s certainly a possibility that they could decide if the data is positive, to launch the product in Japan and that would trigger… milestone payments and sales royalty revenue”.

Other Items of Interest

— I saw a number of comments and received several e-mails regarding the Brotzu lotion. It seems like they updated their EU patent page and several Italian hair loss forum members are claiming that the product is coming out in early 2018. For the time being I will refrain from writing a post on this, as it seems like there is a lot of speculation going on.

— A great thread with before and after photos on Reddit about the power of  the “Big 3” treatment of Finasteride plus Minoxidil plus Nizoral when effective.

PolarityTE starts using its SkinTE product on humans. No info yet on potential hair growth effects on the scalp, but still great to see usage in humans rather than mice so early in the process. Key quote:

“We are confident and believe that SkinTE will replicate its preclinical success and help patients regenerate their own full-thickness, hair-bearing skin”.

Yet Another Dr. Nigam Response

Several weeks after I conducted the interview with Dr. Nigam, he sent me a more detailed reply related to a question I had about why he respects Colin Jahoda’s work so much. Below is Dr. Nigam’s unmodified response:

Regards to my opinion about  Jahoda’s approach and research,I am impressed with him primarily because I think his work has potential application possibility in the “now” and just not in the future. Plus I find his approach street smart, yes I am impressed when he injected his Deramalcupsheeth cells into his wife’s forearms. My approach is similar where in I test various hypothesis on my own scalp and or on consenting individuals, once there are studies confirming the safety of any new protocol. This way like a street smart researcher I can focus on those protocols which shows potential for cure rather than wasting huge money following one protocol for 10 years with the full fledged clinical trial. Any research to MPB cure requires amendments, modifications, additions and subtractions in the protocol as new facts are discovered. Of course the regulations related to Stem Cell research and therapy in India does give an advantage.

If you look back into three major companies who are into research for MPB cure, like Intercystix/aderans, they spent all their money and time into single tunnel view approach i.e isolating Hair Follice Stem cells and culturing them. And in last 10 years so many new facts have come to the fore which they are unavailable to add to their clinical trial, any time they would want to add a new protocol they would need to restart the trial. Same happen Replicell and Histogen and Follica. They all started the clinical trial with Deramalcupsheeth cells, growth factors in hypoxic conditions and FGF9/wounding respectively. But none of the company where no where close to find the cure. As of now my focus is on 3D Spheroidal Trichogenic dp cultures, Trichogenic hair Epithelial stem cells culture, Progenitor bulge stem cells cultures, WNT, SHH, Nogin, FGF 2/5/9, Insulin growth factors, VEGF,EGF,KGF,Follistation etc, Trichogenic freshly isolated dermal papila cells. All the above is in my present protocol. In  the coming months co culturing of epithelial and mesenchymal stem cells and will inc-operate and test other new facts which may improve my protocol as they come into the published domain. 

Nearly everything, even those things that appear original are derivative. Einstein said “The secret of creativity is knowing how to hide your sources.” but i don’t want to hide any sources but want to give due credit to every research whose experimental facts get Incorporated to my protocol. My job is to apply my wisdom like a visionary with street smart commonsense to test what works now on human scalp with an open mind and pour in my efforts, money and time into those line of applied research rather than having a fixed tunnel view research approach. I firmly believe that places like India where Stem cell regulations will be laxed for a year or two. should be the place where leading 8-10 researches should be testing their theories on consented human scalps rather than wasting time and money on lengthy clinical trials. Once the many clinical trials as the process show promise, then the respective companies can go back to their respective places and start their official trials. This what exactly I am doing. And humbly without boasting I have shown results with Hair Multiplications in last 1 year which has not shown by any company on the human scalp through out the globe. I am always open to share my work and collaborate with any like minded researcher or a barter company. Another thing which is common in this industry is people patent everything without testing on single human scalp, once they patent others don’t show interest in that field of patented research as they will not be able to utilize it commercially. And of course the traditional Hair transplant industry gets scared with any success anywhere even partially with HM.