Category Archives: Technion University

Increasing 3-Alpha-Hydroxysteroid Dehydrogenase to Treat Hair Loss?

Update: “Roman” is now answering questions in the comments to this blog post.  At some point I might delete most of the pasted chat messages at the bottom of this post.

Update: I did not realize that the Technion University team from Israel (which I covered briefly last year under 3D printed comb) has a few pages on their website covering this very subject of the 3-alpha-hydroxysteroid dehydrogenase (3α-HSD) enzyme.  See their overview page, cofactor page, comb/design page, expression page, modeling page, secretion page and results page for more.  Looks like they started this work in 2015.


On very rare occasions such as this one, I write a blog post that is almost entirely based upon some random person (typically a passionate intelligent hair loss forum member or e-mailer)’s unique thoughts that I find very interesting and logical, even if I barely understand half of such thoughts unless I am able to devote significant time deciphering various acronyms and scientific terms. Generally, I trust and defer to my intuition in these cases and promote such people and their theories without devoting more than several hours of research unless I really have a lot of free time on my hands.  FYI: An example of such a past recent post:  Intelligent balding men post their theories.

Roman’s First E-mail

On April 16th, a person by the name of “Roman” sent me the following interesting e-mail:

“Hi, below, I am pasting my messages on your chat for what I believe is the most under researched avenue for the hair loss cure and the polar opposite of the approach that finasteride takes.  I think with the power you have you can get this out there as this is something that has been very poorly researched and possibly even hidden. Anyways, enjoy researching but I believe it really does tie the whole pgd2, dht and maybe even wnt pathway approaches under one enzyme: 3-alpha-hsd.”

He ended his e-mail with pasting his prior hair loss chat messages from this site, but I will paste those and other future chat messages that “Roman” made all together at the end of this post since the organization is very haphazard.

Roman’s Second E-mail

I replied to Roman, mostly in the form of an excuse that I could not devote time on research this past week even though I found his thoughts highly interesting.  Below is his second e-mail to me from April 18th:

“Thanks for your reply and I totally understand your work load.  As for a compressed version of my research, as we both know alpha 5ar converts T to DHT.  Well there turns out to be an “antimatter” sort of enzyme present in every human’s musculoskeletal structure called 3 alpha hydroxysteroid dehydrogenase (3a HSD) which converts all T and DHT to 3 adiol g (another testosterone by-product which is not harmless to hair and way less androgenous). Now if propecia can work so well off blocking off alpha 5ar then in theory the exact same should be the case for promoting 3a hsd in synthesis in the body. And finally, the side effects of 3a hsd upregulation should be fractional compared to alpha 5ar downregulation as the reason for the most of the propecia side effects is the lack of DHT being created.  Conversely, DHT will be allowed to generate under this treatment, however, the 3a hsd will instantly de-activate it.  The big hope from my side is that there is a topical way to introduce 3a hsd to the scalp as that will exterminate DHT from the scalp (as it does in the muscles).  Even if there isn’t there’s easily another (potentially a lot safer) variant of propecia waiting to be developed.

A link to start off your research is:

https://thinksteroids.com/steroid-profiles/dht/

Note the paragraph on DHT deactivation in the muscles. Its basically local DHT deactivation which is what everyone has been longing for for decades. It happens everyday in our own body without any negative effects!

Anyways, I hope you find this topic as captivating as I do and I wish you the best of luck on everything to do with your blog and your research on this matter.

Kind Regards,
Roman

PS. if you were on the chat “ihavethecure” “klijom” and “klijomss” were all my names because people kept stealing my name lol!”

Roman sent me two additional e-mails with several useful individual links in there that I discuss below.

My Initial Thoughts and Various Links of Interest

I get many e-mails every week, and typically I only learn something truly interesting from 20-30 percent of them.  Right away I knew that Roman’s e-mail was one of those.  It does seem like increasing the expression of the 3α-hydroxysteroid dehydrogenase enzyme will reduce DHT and lead to a reduction in hair loss and perhaps even lead to regrowth of hair that has been lost in recent years. Perhaps this approach will lead to far fewer side effects than the current approach of using Finasteride or Dutasteride to reduce DHT?

Roman pasted the following 2006 study link in his e-mail that also mentions a PGD2 connection (a difficult read for non-scientists):

Increased expression of type 2 3-alpha-hydroxysteroid dehydrogenase/type 5 17-beta-hydroxysteroid dehydrogenase
(AKR1C3) and its relationship with androgen receptor in prostate carcinoma.

— During my initial research, I found a 2005 thread on hairlosstalk.com on the same subject as well as a 2013 thread on hairlosshelp.com.

In one of Roman’s later chat messages, he posted the following recent 2016 study link that is much more relevant to our hair loss cause and easier to understand in its abstract format:

— Sulforaphane promotes murine hair growth by accelerating the degradation of dihydrotestosterone.

Key quote from above:

“Dihydrotestosterone (DHT) causes the regression of human hair follicles in the parietal scalp, leading to androgenic alopecia (AGA). Sulforaphane (SFN) increases the expression of DHT degrading enzymes, such as 3α-hydroxysteroid dehydrogenases (3α-HSDs), and, therefore, SFN treatment may improve AGA.”

— Another interesting link on Sulforaphane and DHT reduction that someone on the chat posted (could be Roman).  Seems like broccoli has yet one more benefit.

— Finally, on April 18, someone who read Roman’s chat messages on this blog started this useful thread on the hairlosstalk forums that is well worth following.

It seems like antidepressants (SSRIs in particular) can also impact 3-alpha-hydroxysteroid levels…I will write more on that later if there is any interesting potential going that route.

Roman’s Hair Loss Chat Messages

Note that “Roman” has used a few different names in the chat as he mentioned in his e-mail.  One person might have used his chat name with minor modification for some reason (I do not require registration on the hair loss chat on this site).  In any case, I have tried to post only Roman’s chat messages below and those from others that were related to the subject.  In reverse chronological order from the past week with some dates deleted by mistake (4/22/16 –> 4/16/16):

asfasas 4/22/2016 3:10
“conclusion kijom ??”

I am not yet at the conclusion I am still actively researching the matter. What I have discovered though is that 3a hsd is an enzyme which is located in various parts of the body where it deactivates DHT. Now I am researching there is sufficient presence of 3a hsd in the scalp and if not, whether there are viable ways to introduce it to the scalp.

2020 4/21/2016 9:42
Can someone please simplify this for me

2020 4/21/2016 9:42
This is so confusing what you guys are talking about.

yazed 4/21/2016 7:41
conclusion kijom ??

klijom 4/21/2016 4:33
okay idk why its putting an emoji in it but I promise it works lol

klijom 4/21/2016 4:33
http://press.endocrine.org/doi/full/10.1210/en.2002-0032?view=long&crazymid=12810547&

klijom 4/21/2016 4:33
extremely interesting article for the (medically aware) people to read : http://press.endocrine.org/doi/full/10.1210/en.2002-0032?view=long&crazymid=12810547&

lmlmlm 4/21/2016 4:10
type 3 3a hsd **

lmlmlm 4/21/2016 4:09
basically whoever is researching this needs to find a way of upregulating akr1c2, or more specifically type 3a hsd in the scalp.

lmlmlm 4/21/2016 4:04
Thinking behind Ursodeoxycholic acid is that it is an akr1c2 upregulator but I am trying to see if it has any scalp benefits.

lmlmlm 4/21/2016 4:01
as a starting point in 3a hsd boosting medications is sulforaphane. I have just ordered some. Also if anyone has the medical capacity to research Ursodeoxycholic acid, that would be great as I keep running into walls.

jkjnk 4/21/2016 10:17
Localised deactivation of DHT happens all over your body; in your muscles, brain, liver etc etc. Now we just have to find a way to introduced localised deactivation in the scalp. Peace xoxo

jkjnk 4/21/2016 10:15
Looking deeper into the highest expressions of 3a hsd, you discover that in your muscles, DHT levels have no androgenic effects whatsoever, and this is all due to the constant life long deactivation of DHT by 3a hsd. Now that is the goal I am aiming for in the scalp; a life long deactivation of any DHT that comes into the scalp by 3a hsd without feminising the body the way that propecia does.

jkjnk 4/21/2016 10:12
The main problem why SSRI’s arent cropping people with full heads of hair is because the conversion of DHT to 17 b adiol is a reversable on. Therefore any dht converted to to 17b adiol will later be converted back to dht by alpha 5ar present in the scalp if there is no 3a hsd present.

jkjnk 4/21/2016 10:09
Also a pattern I noticed, no research behind this, is that morbidly obese men more often than not have full heads of hair. Curiously looking deeper in to this, I discovered that obese men express more 3a hsd in their systems http://joe.endocrinology-journals.org/content/191/3/637.full.pdf

jkjnk 4/21/2016 10:07
There is so little research on 3a hsd. 3b hsd deficiency is topic that is researched way more. Basically type 3 3a hsd is the enzyme we are after and that comes under the akr1c2 gene. Now I am currently in the process of identifying how well 3a hsd is expressed in the scalp (if at all). The goal is to bring increased akr1c2 (increased type 3 3a hsd) expression to the scalp.

Klijomm 4/20/2016 11:08
i read of a 5 fold increase in 3ahsd activity following levothyroxine treatment in trial. any idea how this might relate to activity at the scalp? would any ssri amplify considering net impact? http://www.jle.com/en/revues/ejd/e-docs/steroidogenic_enzymes_in_skin_100522/article.phtml

kilojom 4/20/2016 3:08
Hi test. I have seen the post and will make an account to comment

ok 4/20/2016 4:37
klijommm, it’s me, “test” (I had to change my username in the chat). I made a HLT thread about 3a hsd the other day and people think it is really interesting. It would be great if you made an account and joined the discussion over there, since you have a lot of knowledge about this.

klijommm 4:43
Also this is bearing in mind that there is little 3a hsd present in the scalp and people are still noticing an improvement in their hair. Now if we could introduce higher amounts of 3a hsd to the scalp then i think that is game over rip gg for the hair loss industry. (PS stop stealing “klijom” lmao)

klijommm 4:41
http://www.webmd.com/vitamins-supplements/ingredientreview-1070-SULFORAPHANE.aspx?drugid=1070&drugname=SULFORAPHANE

klijommm 4:41
Sulforaphane is currently the best avenue for 3a hsd promotion in the body. Check the review (on a totally non hair loss related website) from the lady who is talking about its effects on her hair.

klijomm 2:05
if anyone wishes to get in touch with me regarding any 3a hsd matters pls email me at romankristofsenwp@hotmail.com (please dont spam lol I’ll just delete the account)

klijomm 1:28
@m also equol is a non steroid estrogen whereas 3a hsd directly converts DHT to a less androgenous derivate of testosterone (not an estrogen) hence your point holds no substance

klijomm 1:27
@m here is a link to offer you some insight into the work that 3a hsd does and where it is present

— https://thinksteroids.com/steroid-profiles/dht/

klijomm 1:27
@m yes SSRI’s do increase 3a hsd activity but the problem is that there is not enough 3a hsd present in the scalp. Also SSRI’s are not designed to increase 3a hsd activity, it is merely a side effect. If a drug/method could be developed to introduce 3a hsd to the scalp then we are on to a real winner

klijomm 1:24
@test I would be more than happy for you to take over the topic and get it onto the HLT forum as I am not a member on any forum website. I really do appreciate your interest in the topic however and can assure you it (although it is difficult due to the lack of research done on the matter) it is totally worth researching!

test 3:51
klijom, 3a hsd sounds very interesting, I really hope that you are onto something here

test 3:49
Or else I’ll do it to get your word out there, I believe what you’re saying is very important! I’ll credit you of course

test 3:44
killjom, could you please make a thread on the HLT forum (new research, studies and technology) about this 3a hsd enzyme? That would help a lot

hujk 5:14
this 3a hsd topic sounds really interesting, especially the anti matter comparisons with alpha 5ar.

klijom 3:08
the best chance of a cure coming out is if someone takes 3a hsd seriously and finds a way to introduce it to the scalp. If I had any sort of background in biomedical science I would be working on it right now as it does not seem too difficult to introduce enzymes to the scalp (no chance of rejection if enzymes developed are taken from your own body) and virtually no chance of mutations (cancer).

klijom 7:38
There seems to be a recently formed small team in Israel called Technion who have also identified 3a hsd as the main culprit. It is interesting to see their valiant but a little amateurish experiment results.

klijom 7:24
… to block alpha 5ar. WE SURE AS HELL can create something to magnify 3a hsd activity. And it is my understanding that formulating a topical treatment is a million times easier when you are trying to boost a function than inhibit (hence the difficulties in formulating a topical propecia).

klijom4 7:22
Also check this out, isoretinoin, better known as accutane is a drug infamous for causing hair loss. the reason? Isotretinoin causes upregulation of D2 receptors. Upregulating D2 receptors is bound to lead to hair loss (see Dr Cotsarelis). Another side effect of isoretinoin?? IT INHIBITS 3A HSD ACTIVITY…..! It’s clear that the antimatter to alpha 5ar is indeed 3a hsd and if we can make propecia

klijom4 7:11
SOMEONE NEEDS TO GET THIS OUT THERE ITS THERES AN ANTI PROPECIA OUT THERE SOMEWHERE AND THE BEST PART IS IT WONT GIVE US LIMP d**k LOL

klijom4 7:03
I really hope the admin sees this and decides to address it in some form in the next article.

klijom4 7:02
…be at least a permanent topical (worst case scenario oral) treatment. The link of 3a hsd and prostate cancer again seems to solidify this claim as this is the exact route that propecia and alpha 5ar inhibition came from.

klijom4 7:01
Also it seems that many of the “hair loss fads” that we hear about e.g. coconut oil, olive oil etc may actually be increasing 3a hsd activity (in however small effect). Now if this effect can be harnessed and magnified which may be a lot easier than most of the other methods being developed at the moment (follica histogen etc) then we will have something that although not a cure but would

klijom4 6:57
…be a lot easier than trying to remove an enzyme from a scalp (alpha 5ar). I honestly am starting to believe this a massive avenue which has been completely ignored and I don’t see why (ethics aside) 3a hsd supplements for the scalp could not be created to “neutralize” dht as soon as they interact (as they do in the muscles).

klijom4 6:55
Also that thread, is more than ten years old and I tried googling 3a hsd to find only 2 or 3 semi-serious discussions of it regarding hairloss. 3a hsd is a natural enzyme present within the muscular structure of the body which prevents the conversion of testosterone to dht wherever it is present. Now to me it would seem that introducing an enzyme to the scalp (3a hsd) should in theory…

klijom4 4/16/2016 6:52
@bigt1 I think he does have a point though. Why try and fight alpha 5ar when you have a natural mechanism in your body designed to do just that? It shocks me how poorly this issue has been (or not been) covered.

bigt1 4:49
http://www.hairlosstalk.com/interact/showthread.php/15421-3a-HSD-(3alpha-hydroxysteroid-reductase)

@Ihavethecure this where your getting your info from?

@Ihavethecure 4/16/2016 2:54
hair loss is being researched by monumental retards who are taking all your money.

Ihavethecure 21:54
article linking 3a-hsd with the long heralded pgd2 cure branded about by cotsarelis.

Ihavethecure 21:53
http://erc.endocrinology-journals.org/content/13/1/169.full.pdf

Ihavethecure 21:44
It’s way easier (and safer) to augment 3a-hsd levels rather than inhibiting 5alpha reductase levels.

Ihavethecure 21:35
SSRIs fluoxetine, fluvoxamine, sertraline, and paroxetine, the SNRI venlafaxine, and mirtazapine. All current treatments used as anti depressants to increase 3a-hsd levels

Ihavethecure 21:29
Anyways, that’s your cure, go spread the word, took my a*s long enough to find it. I don’t know how to produce a treatment to administer the appropriate therapy but if testosterone therapy can overcome 5-alpha reductase deficiency then the exact mechanisms with a different drug make up would provide adequate doses of 3a-hsd to the scalp.

Ihavethecure 21:25
All the pharmaceuticals know about 3a-HSD and the natural DHT eliminating property that it has. But then again, replicating an enzyme is something only the very top pharmaceuticals can do and they wouldn’t want to lose such a steady stream of revenue lol.

BiologicsMD, 3D Printed Comb and Thicker Eyebrows

Hair loss news first:

BiologicsMD

On July 30, it was announced that the University of Arkansas (along with several other entities) was issued a patent for a new hair loss drug based on work done by Dr. Joshua Sakon and three others. The patent is titled “Fusion Proteins of Collagen Binding-Domain and Parathyroid Hormone.”

Arkansas based privately held BiologicsMD holds the exclusive license to this technology. Their related hair loss drug will be known as BMD-2341. The related patent issued by the US Patent and Trademark Office is No. 9,062,300. The main portion of BiologisMD’s work entails bone growth, including for the spine, fracture repair and osteoporosis.

3D Printed Comb from Technion University

I am surprised that there has not been much new research coming from Israel when it comes to hair loss. The country has a booming start-up scene, and from my observations, Jewish people seem to suffer from baldness at an even higher rate than Caucasians (who in turn have much higher rates of baldness compared to Asians). In any case, this new 3D printed comb for hair loss project from Technion University in Israel seems interesting (you need to translate), although I would not be surprised if we never hear about it again. FYI — For any readers in Israel, here is an article from 2011 with names of local hair loss experts and clinics that you could consult.

Lumigan for Thicker Eyebrows

For several months, Spencer (aka Spex) has been experimenting with using Bimatoprost on his previously sparse eyebrows. He uses the Lumigan brand that is designed to reduce high pressure in the eyes. He recently added the above page on his site, and it is well worth checking out the before and after photos on there. Many hair loss sufferers have been waiting for months to hear about the delayed results of the clinical trials of Bimatoprost when used on scalp hair. While I have been skeptical that the drug will do much beyond what Minoxidil already does for scalp hair, Spex’s eyebrow results are very encouraging. Bimatoprost, if approved for use on the scalp, will entail a drastically higher dosage compared to what is used on eyebrows.

Other Hair Loss News this Month

— A very interesting radio interview with Dr. Luis Garza regarding his team’s latest groundbreaking findings on triggering organ and hair regeneration. See my recent post on those findings.

Samumed is recruiting for a 50-person supplemental clinical trial for its SM04554.

— George Cotsarelis gets yet one more patent approved (this one related to FGF-9 and hair growth). Filed in October 2014 and approved in August 2015.

— An optimistic conclusion from a molecular biologist: “In any case, I think that treatment for baldness is now a matter of quite a short period of time.” Article rambles a bit, perhaps because the writer is not a native English speaker.

— Some interesting thoughts on platelet-rich fibrin matrix from Dr. William Lindsey.

— In June, Dr. Alan Feller started a controversial thread on the HTN forum regarding strip (FUT) hair transplants still being more popular than FUE hair transplants. That thread has taken on a life of its own, and I only read it this month since I do not frequent those forums too often. Based on my own research (see FUE versus FUT hair transplants), I do not believe that strip will remain very popular. It already might be less popular than FUE, considering that doctors can now just purchase the ARTAS robot. They can then start practicing FUE with little past experience in doing so. In any case, Dr. Feller raises some interesting points in that thread, and I wonder if FUE transaction rates are really that high in the hands of experts? If I was getting a hair transplant today, I would go for FUE.

— A very useful update from a Japanese resident regarding AAPE and HARG treatment in Japan.

— An interesting study (on mice), where pluripotent stem cells from whisker follicles differentiate and grow into new hair when transplanted to the spinal cord.

— Comedian Matt Lucas has suffered from alopecia universalis for most of his life. A nice story on him helping a young boy suffering from the same here.

— Somewhat related to the above, scientists use cells created from hair follicles to repair damaged nerves.

And now on to medical items of interest:

Things are getting creepier and creepier and at the same time evermore mind-boggling each month.

Nearly complete brain developed in petri dish by Ohio State scientists. This was major news yesterday and today.

— United Therapeutics (Revivicor) is genetically engineering pigs in order to transplant their organs into humans. I find it absolutely fascinating that you can insert human genes into animals and that scientists are able to increase the number that they can insert every year. The founder and CEO of United Therapeutics is the amazing MTF transsexual Martine Rothblatt, who also co-founded Sirius XM satellite radio.

Young blood is what we all need.

Body-hackers. Worth clicking just to see the image.

— A pro designer baby article worth a skim-through. The Chinese will probably stab at this first.

A list of the top 11 3D-Bioprinting companies.